Rekombinanter Topoisomerase II alpha Antikörper (AA 1352-1493)

Produktnummer ABIN7127009

Dieser Anti-Topoisomerase II alpha Antikörper ist ein Maus Monoklonal Antikörper zur Detektion von Topoisomerase II alpha in WB und IHC (f). Geeignet für Human.

Kurzübersicht für Rekombinanter Topoisomerase II alpha Antikörper (AA 1352-1493) (ABIN7127009)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: AA 1352-1493

Spezifität: It recognizes a 170 kDa protein, which is identified as topoisomerase IIi±. It shows no cross-reaction with Topoisomerase II i² or I. Topo II i± plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II i± is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Kreuzreaktivität (Details): Human. Mouse.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human Topoisomerase II alpha fragment (aa1352-1493)

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Jurkat cells. Human lymph node or tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: TOP2A

Hintergrund: ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, DNA Topoisomerase2, TOP2A, Topoisomerase DNA II alpha 170 kDa, TP2A,Topoisomerase II alpha (Proliferation & Drug-Resistance Marker)
Cellular localisation: Nuclear

Molekulargewicht: 170kDa

Gen-ID: 7153, 156346

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases